Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: MTT assay for determining cell viability. MTT assay was performed on both A2780 and NIH/3T3 cells to determine cell viability. Cells were treated with a chemical compound called harmaline at different concentrations for 24 and 48 h. The degree of tetrazolium reduction observed in each cell line would be an indicator of the number of viable cells in the culture.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: MTT Assay

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: (a) Harmaline induced apoptosis in ovarian cancer cells. The apoptosis of A2780 cells was measured after 24 h harmaline treatment (150 and 300 μM) by flow cytometry. Live cells, early apoptotic cells, late apoptotic cells, and necrotic cells are each represented by Q4 to Q1 in the diagram. (b) The late apoptosis and necrosis were significantly increased dose dependently. The individual data points and significant p ‐values are shown in each column, and the data are represented as mean ± SD.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: Flow Cytometry

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: Harmaline induced the expression of Bax and p53 and reduced the expression of MMP2 and MMP9 at the transcription level of ovarian cancer cells. A2780 malignant ovarian cancer cells were individually treated with 150 and 300 μM of harmaline for 24 h, and then the cells were harvested for RT‐PCR to quantify the gene expression of the Bax/Bcl‐2 ratio, p53, MMP‐2, and MMP‐9. The results are presented as means ± standard deviation. The individual data points and significant p ‐values are shown in each column.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Standard Deviation

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: Harmaline induced ROS production in A2780 cells in vitro. (a) After a 24‐h period, we examined the impact of harmaline on the generation of reactive oxygen species (ROS) in A2780 cells using DCFDA as a fluorescent dye. The production of ROS in A2780 cells showed a significant increase when treated with 300 μM of harmaline. However, when N ‐acetyl cysteine (NAC) was administered along with harmaline, there was a notable inhibition of harmaline‐induced ROS production. As a positive control, tert‐butyl hydroperoxide (TBHP) was introduced to A2780 cells, which resulted in a significantly higher ROS production compared to the control. (b) Combining N ‐acetyl cysteine (NAC) with harmaline at concentrations of 300 μM resulted in enhanced viability of A2780 cells 24 h after treatment, as compared to each group individually with the same concentrations. The results are expressed as means ± standard deviation. The individual data points and significant p ‐values are shown in each column.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: In Vitro, Inhibition, Positive Control, Control, Standard Deviation

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: Effect of harmaline on the migratory capacity of A2780 cells in vitro. The migratory ability of harmaline‐treated A2780 cell line cells was analyzed using a wound‐healing assay. (a) Migration was monitored at 2 and 48 h. (b) The migration capacity of A2780 cells was significantly impaired by harmaline (37.5 and 75 μM) treatment compared to untreated control. The individual data points and significant p ‐values are shown in each column, and the data are represented as mean ± SD.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: In Vitro, Wound Healing Assay, Migration, Control

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: Harmaline decreased the MMP‐9 and MMP‐2 secretions of A2780 cells in vitro. The zymography test was performed to evaluate MMP‐2 and ‐9 activity. The bands represent the digested areas of gelatin by MMP‐9 and MMP‐2 after 24 h harmaline treatment (37.5 and 75 μM). The results showed that relative enzyme activities as a percent of the blank group for MMP‐2 and MMP‐9 significantly decreased after the treatment of A2780 cells with harmaline. Data presented as relative MMP‐9 and MMP‐2 activity versus untreated cells. The individual data points and significant p ‐values are shown in each column, and the data are represented as mean ± SD.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: In Vitro, Zymography, Activity Assay

Journal: Physiological Reports

Article Title: Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

doi: 10.14814/phy2.15984

Figure Lengend Snippet: Harmaline inhibits the invasion of A2780 cells in vitro. (a) The impact of harmaline on the invasion of A2780 ovarian cancer cells was evaluated by treating the cells with 37.5 and 75 μM of harmaline for 24 h in media containing serum. (b) The invasive cells were then assessed using the cell invasion assay QCM™ 24‐Well Colorimetric Cell Migration Assay. The results showed that the number of invasive cells was significantly lower after treatment with harmaline compared to the control group, with values expressed as means ± standard deviation. The individual data points and significant p ‐values are shown in each column.

Article Snippet: The human ovarian carcinoma cell line A2780 and the NIH/3T3 cell line were both purchased from the Iran Pasteur Institute in Tehran, Iran.

Techniques: In Vitro, Invasion Assay, Cell Migration Assay, Control, Standard Deviation

Journal: Stem Cells International

Article Title: Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

doi: 10.1155/2016/1652389

Figure Lengend Snippet: Characterization of DCV-SP cells using pharmacological inhibition and antibody-based costaining of SP-conferring drug transporters. A2780V cells containing ABCB1-positive SP (a) and parental A2780 cells harbouring an ABCG2-positive SP (b) were stained with 10 μ M DCV (10 6 cells/mL) and subjected to a set of control experiments. Specifically, the samples were inhibited using 50 μ M verapamil (blocking several drug transporters) or 20 μ M fumitremorgin C (blocking ABCG2 specifically) or, where indicated, both. In addition, noninhibited and inhibited cells were costained for the drug transporters ABCB1 and ABCG2 using respective monoclonal antibodies. This multimodal approach helped us to better define particularly A2780V cells, whose SP contains a major population of ABCB1-positive cells (~8–10%) and a minor population of ABCG2-expressing cells (~0.1%). Note that, at least in our model systems, verapamil does not inhibit the activity of ABCG2.

Article Snippet: The A2780 human ovarian carcinoma cell line was purchased from Sigma-Aldrich (Catalog number 93112519), and A2780V cells, a subline of A2780 [ ], were kindly obtained from Professor Zeillinger, Vienna, Austria.

Techniques: Inhibition, Staining, Blocking Assay, Expressing, Activity Assay

Journal: Stem Cells International

Article Title: Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

doi: 10.1155/2016/1652389

Figure Lengend Snippet: DCV does not impinge on cell proliferation or single cell clonogenicity. (a) A2780V cells were stained with 10 μ M DCV (10 6 cells/mL) and SP/NSP fractions were flow sorted. Equal numbers of cells were then cultured and cell proliferation was assessed after 48 h using 3 H-thymidine incorporation. Under these conditions, dye-retaining NSP cells proliferate equally well as their dye-extruded SP counterparts. (b) Comparative single cell cloning of freshly sorted (“+DCV”) versus presorted, in vitro expanded SP/NSP cells (“−DCV”). In two investigated cell lines (i.e., A2780 and IGROV1), no notable differences could be detected between the two experimental setups, indicating that the presence of DCV does not affect the clonogenic capacity of NSP cells.

Article Snippet: The A2780 human ovarian carcinoma cell line was purchased from Sigma-Aldrich (Catalog number 93112519), and A2780V cells, a subline of A2780 [ ], were kindly obtained from Professor Zeillinger, Vienna, Austria.

Techniques: Staining, Cell Culture, Clone Assay, In Vitro

Journal: Stem Cells International

Article Title: Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

doi: 10.1155/2016/1652389

Figure Lengend Snippet: SP detection using the second-generation DyeCycle dyes DCG and DCO. A2780V cells harbouring an ABCB1-positive SP (a) and parental A2780 cells containing an ABCG2-positive SP (b) were stained with 500 nM of either DCG or DCO (10 6 cells/mL) and analysed by flow cytometry using the indicated emission filters (DCO data accentuated by dotted lines). For control purpose, verapamil (A2780V) or fumitremorgin C inhibition (A2780) was performed, and drug transporters were stained with APC-conjugated monoclonal antibodies. Both DCG and DCO are able to detect ABCB1-positive SP cells, whereas neither dye can identify SP cells that express ABCG2. Note the extreme separation of ABCB1-SP cells, which made it impossible to measure the fluorescence linearly.

Article Snippet: The A2780 human ovarian carcinoma cell line was purchased from Sigma-Aldrich (Catalog number 93112519), and A2780V cells, a subline of A2780 [ ], were kindly obtained from Professor Zeillinger, Vienna, Austria.

Techniques: Staining, Flow Cytometry, Inhibition, Fluorescence

Journal: Inorganic Chemistry

Article Title: Hydrolytically Stable and Cytotoxic [ONO N ] 2 Ti(IV)-Type Octahedral Complexes

doi: 10.1021/acs.inorgchem.2c02737

Figure Lengend Snippet: Dependence of human ovarian A2780 (top) and colon HT-29 (bottom) cancer cell viability on different concentrations (shown on a logarithmic scale) of L 1–4 2 Ti, following a 3 day incubation period as analyzed by the MTT assay. Cisplatin IC 50 = 1.6 ± 0.4 μM for A2780 and IC 50 = 25 ± 4 μM for HT-29. H 2 L 1–4 = 1.9 ± 0.7, 1.5 ± 0.1, 1.4 ± 0.6, 7.4 ± 1.5 μM for A2780 and IC 50 = 1.3 ± 0.4, 10 ± 3, 10 ± 3, 26.9 ± 6.2 μM for HT-29 (see maximal inhibition values in Table S1 ). Relative IC 50 values were calculated by nonlinear regression of a variable slope model by GraphPad Prism 5.04 software ([top + bottom plateaus]/2).

Article Snippet: The cytotoxicity of Ti(IV) complexes was tested on A2780 human ovarian carcinoma cell line (European Collection of Authenticated Cell Cultures) and HT-29 human colorectal adenocarcinoma cell line (American Type Culture Collection).

Techniques: Incubation, MTT Assay, Inhibition, Software